Chapter I: Preface: To the Second Edition

In preparing this edition I have endeavoured to meet the requirements of students, and of practitioners who desire to keep up their histological work. Those methods are selected which have been found to work well in practice, and it has been thought better to describe a few in detail rather than give a short account of many similar methods.

I have again to express my obligation to the various instrument makers for the illustrations of microtomes, &c.; to Dr. Fearnley, of Bradford, for the description of his method for injecting blood vessels, and to Messrs. Macmillan and Co. for permission to copy figures 10 and 11.

W. S. COLMAN.

Wimpole Street, W.
_Sept., 1896._

CONTENTS. PAGE

CHAPTER I.
Apparatus Required 1

CHAPTER II.
Hardening Processes 15

CHAPTER III.
Section Cutting 29

CHAPTER IV.
Section Mounting 55

CHAPTER V.
General Staining Methods 67

CHAPTER VI.
Special Methods for Staining the Nerve Centres 87

CHAPTER VII.
Special Methods for Staining Micro-Organisms and Blood 103

CHAPTER VIII.
Injection of Blood Vessels 120

CHAPTER IX.
Directions for Preparing Individual Tissues 129

Index 153

SECTION CUTTING AND STAINING.