Chapter XXVII: Part III: Scientific Pursuits (5)

Another point which calls for extreme attention is the perfect cleanliness of the glasses. It is astonishing how a tiny dust-mote, or the least condensation of damp, will diminish the powers of the microscope, and how often the instrument is blamed for indistinctness, when the real fault lies in the carelessness of the operator. Even when the greatest care is taken, dust is sure to settle on the glasses, especially on the eye-piece, and before using the microscope the glasses ought to be carefully examined. Never wipe them with an ordinary handkerchief, but get a piece of new wash-leather; beat it well until no dust issues from it, and then put it into a box, with a tightly-fitting cover. Use this, and nothing else, for cleaning the glasses, and you will avoid those horrid scratches with which the eye-glass and object-glass of careless operators are always disfigured.

Moisture is very apt to condense on the glasses and to ruin their clearness. If the microscope be brought from a cold into a warm room, the glasses will be instantly covered with moisture, just as the outside of a tumbler of cold water is always covered with fine dew when brought into a warm room. The microscope should therefore be kept at least an hour in the room wherein it is to be used, so that the instrument and the atmosphere may be of the same temperature. You should make the microscope a trifle warmer than the surrounding atmosphere, and so avoid all danger of condensation. When changing the object-glass or eye-piece, always keep the hand as far away from the glass as possible, and manipulate with the tip of the forefinger and thumb. The human skin always gives out so much exhalation, that even when the hand is cold the glasses will be dimmed; and it is a peculiarity of such moisture, that it adheres to the glasses with great pertinacity, and does not evaporate like the dew which is condensed from the atmosphere.

In order to insure perfect success in this important particular, the young microscopist will do well to get the optician from whom he purchased his instrument to explain its construction, and to give him a lesson or two in the art of taking it to pieces and putting it together again; for unless each glass can be separately cleaned, no one can be quite sure that the instrument will perform as it ought to do. The best method of ascertaining whether it is quite clean is to throw the light upwards by means of the mirror, and then to turn the eye-piece slowly round. If any dust or moisture has collected either upon the eye-glass or the “field-glass,” which forms the second lens of the eye-piece, it will be immediately detected. Turning the object-glass will in a similar manner detect impurities upon its surface.

We will now proceed to the manner in which objects are examined. Suppose, for example, that we take a buttercup-leaf, because it can be found at almost any time of the year. Place a piece of glass on the stage, lay the leaf on it, put on the lowest power, set the focus, and then look at the leaf. You will probably be disappointed, and see nothing but a confused mass of undulating dark green, like a green carpet thrown carelessly on the ground, and seen in the dim twilight.

Two points are now needed; the first being to get the leaf flat, so as to avoid the undulation, and the second being to throw a proper light upon it.

Take out the leaf, and, instead of laying it entire under the microscope, select the flattest part, and cut it out with scissors. A piece the size of a silver penny will be amply large enough. Lay this piece on the glass, get the focus afresh, and then look through the microscope. The leaf will now appear much more regular, and will be seen as a rough surface, mottled with white and traversed by pink and green ridges, which are the large and small nervures. By means of a mirror or the condenser throw a brighter light upon it, and it will be seen to be covered with a slight roughness, the nature of which cannot be clearly ascertained; then add the next highest power, and try if the structure of that roughness can be made out. Curiously enough, although the magnifying power has been more than doubled, the roughness has much the same appearance as before; so that we must try another plan, and look at the leaf edgeways.

Take the piece of leaf in the stage-forceps, but _do not touch it with your hand_; fix the forceps on the stage and turn the leaf so that it presents its edge to the object glass. Get your focus, and you will now see the cut edge of the leaf, and will at once distinguish its structure. On either side may be seen the upper and lower cuticle, and in the centre the soft green substance, or “parenchyma,” as it is called. From the cuticle project a number of short hairs, and when the focus is accurately obtained, the cause of the roughness will be seen in a vast number of minute projections, which are, in fact, identical in structure with the hairs, though not so well developed. The under-cuticle of the leaf is much more interesting than the upper.

Now change the illumination, and, instead of throwing the light upon the object from above, turn the mirror so as to direct it through the object from below. No apparent result will follow, because the leaf is so thick and opaque that the light cannot pass through it. Hold the leaf horizontally, and, by means of the stage-forceps, rip it smartly across, and if you do this rightly, you will find that the two cuticles are partly separated, so as to allow either to be examined separately. At first the leaf will most probably be torn along one of the large nervures, so that the cuticles are not perfectly separated. Never mind failure, but try again; and you are sure, after a few efforts, to hit upon the right method of tearing the leaf.

One of the most useful capabilities of the “live-box” is now shown. As may be seen by the figure and section, it consists of an inner tube with a thick glass, and an outer tube with a thin glass. The outer tube can be taken off, water or any other substance laid on the thick glass, and then the outer tube or cover is slid down upon it until the object is pressed flatly between the two glasses. When you have succeeded in getting a convenient slip of the leaf, lay it on the thick glass of the inner tube, and put a drop of water on it. Put on the cover, and push it down until the piece of leaf is pressed flat, without being squeezed. Now look through the microscope, and you will see a beautiful sight, showing how much there is in a despised leaf, which we daily tread under foot.

The cells of which the cuticle is chiefly composed are seen in many a waving outline, while at their points of junction are placed the remarkable contrivances called “stomata,” or mouths, which are the apertures through which the atmosphere is enabled to penetrate into the interior of the leaf. The two semilunar cells at the sides of the opening may be considered as lips, which open and close according as the plant needs the air or not. The numerous dots which are seen upon the leaf are of a vivid green colour, and it is to their presence that the leaf owes its hue.

We have given these details because they are applicable to the examination of all leaves and petals, and show the young observer the method which is to be adopted when looking for the first time at a strange object.

If the microscopist should follow up his work properly, and make sketches of every object which he places under the microscope, he cannot do better than use the camera-lucida, a neat little instrument, which is fitted into the eye-piece of the microscope. Dr. Beale’s neutral glass is as efficacious in careful hands, and only costs a fourth of the sum. This instrument cannot be applied to the ten and twenty shilling microscopes, as it requires that the tube should be perfectly horizontal. The method of using it is simple enough.

After fixing the object and getting the right focus, set the instrument horizontally, and arrange the light so that the object is well illuminated, and its lines quite clear and well defined. Now remove the cap of the eye-piece, and fix the camera-lucida in its stead. Lay a drawing-pad on the table under the camera-lucida, look through the square opening (or, if you use Mr. Beale’s glass, look through the neutral glass), and you will see the object apparently projected on the paper. We say apparently, because in reality the image is not thrown on the paper at all, but on the camera, and the eye refers it to the paper, as being the nearest object. In fact, the principle on which this camera-lucida is arranged is exactly that of the Polytechnic ghost, which appears to be in one place, whereas it is in another.

Now take a pencil, cut it to a very fine point, and trace the outline of the object on the paper. At first you will think this to be an impracticable task, for the point of the pencil will totally vanish. Soon, however, the eye will so adjust itself as to see the pencil and the object perfectly well, and by a little practice the observer will be able to sketch every object as rapidly and firmly as if he were copying a drawing, by means of tracing-paper. The neutral glass is perhaps to be preferred to the camera-lucida, as it is learned more easily, and gives less trouble than that instrument. Its cost is five shillings.

After you have practised yourself well in the handling of the microscope, your ambition will take another step, and lead you to the preparation of permanent objects. In order to set yourself up with the needful apparatus, you will have to disburse about five shillings. A small spirit-lamp will cost eighteenpence, and a small bottle of Canada balsam, another of asphalte varnish, and another of Dean’s gelatine, will make about eighteenpence or two shillings more. A few pence will purchase a sheet or two of ornamental paper, and a few more a flat plate of brass or copper, about five inches by three. The rest of the five shillings may be expended in “slides” and thin glass, cut square.

Slides are merely slips of glass, three inches in length by one in width, and the thin glass is used for laying upon the objects and defending them from dust. We advise the square glass, because it scarcely costs one quarter as much as the round glass, and is equally effective when properly managed. There are several methods of “putting up” preparations--namely, dry, in Canada balsam, in gelatine, and in cells. We will take them in their order.

The simplest plan is, of course, the “dry” mode. Suppose that you want to preserve a tiny piece of down, or the scales from a butterfly’s wing. First wash all the slides and glasses well, by dipping them into a strong solution of soda, and then into hot water, in order to get rid of grease, taking care never to touch them with the hand, but to take them out of the water with the forceps. This can be done at any time, and the glasses carefully wrapped up and placed in a box ready for use.

You now select one of the slides, and lay the object exactly in its centre. If very minute objects are used, they must be examined in order to see whether they are properly disposed. The next process is, to take one of the thin glasses with the microscope, and lay it very carefully over the object. Then cut a piece of ornamental paper, about two inches long and seven-eighths of an inch in width; cut or punch a circular piece out of its centre, damp it well, and cover the wrong side slightly, but completely, with paste. Lay it on the slide, so that the centre of the hole shall coincide with that of the object, work it down neatly with the fingers, and it will hold the square piece of thin glass, which is technically called the “cover,” in its place. Watch it occasionally as it dries, and be ready to press down any part of the paper that may start up. Write, with ink, the name of the object on the end of the slide.

When you have made a dozen or two of these preparations, it will be time to letter and index them. On each slide paste a slip of white paper, and on the paper write a brief notice of the object, thus--

+---------------+
| SCALES. |
| |
| D. HEAD MOTH. |
+---------------+

Then scratch with a bit of flint, or with a writing-diamond, if you have one, a number on the end of the slide, and have a note-book with a corresponding number opposite to which you enter the description at a fuller length, thus:--

18--Scales of Death’s Head Moth (_Acherontia Atropos_), from centre
of under-surface of right fore wing. Dry. June 4, 1864. +

The cross signifies that you prepared the object yourself, and the reason for adding the date is, that in after years you will have a valuable guide as to the durability of your preparations. If the specimen has been purchased or presented, always add the name of the seller or donor, as well as the date. These precautions may seem to be needlessly minute, but we have so often seen whole sets of valuable preparations rendered useless for want of ticketing, that we cannot too strongly impress on our readers the necessity for the note-book as well as the label, the one acting as a check upon the other. When the label has been affixed, and the details transferred to the note-book, the ink may be washed off the end of the slide.

There is another convenient method of putting up the elytra of beetles, parts of various insects, mosses, minute shells, and similar objects. Take a common pill-box of the smallest size, and cut a little cylinder of cork, that will nearly, but not quite, equal the height of the box, and fasten one end to the bottom of the box with glue. Now blacken the interior of the box and the cork cylinder. Put a little drop of Canada balsam, Arabian cement, or gum Arabic on the top of the cylinder; put the object on it, press it into its place, and, when the cement is hard, the preparation is complete. The cover of the box serves to keep the object from dust.

Now we come to the Canada balsam, a substance which produces beautiful effects when rightly handled, but is most aggravating to the learner, causing alternate irascibility and depression of spirits. Many objects, such as the antennæ and feet of insects, will not show their full beauty unless they are mounted in Canada balsam. The method of doing so is as follows:--A week or two beforehand put the objects into ether or spirits of turpentine, and allow them to remain there until wanted. Pile up some old books, or take a couple of convenient wooden blocks; lay your brass plate upon them; light the spirit-lamp, and put it under the plate so as to heat it. Lay two or three slides on the plate, and all then can be heated at the same time.

Warm the bottle of Canada balsam, and with a glass rod take out a very little drop, and put it exactly in the middle of the slide. In order to insure this point, I always put a dot of ink on the wrong side of the slide. Stir it about with one of the needles mentioned on page 428, and if any bubbles rise, break them. When the balsam is quite soft and liquid, take one of the objects out of the bottle and put it into the balsam, exactly over the black dot. Now add a little more balsam, so as to cover it, and let it lie for a few moments. Take one of the glass covers, put a very little balsam on its centre, and lay it neatly over the object, pressing it down gradually and equally. Unless this be done, the object will not remain in the centre, but will shoot out on one side, and the whole operation must be begun _de novo_. Remove it from the hot plate and lay it on a cool surface, still continuing the pressure until the balsam has begun to harden. Lay a little leaden weight--a pistol-bullet partly flattened is excellent for the purpose--and on the cover write the name of the object, as already mentioned, and then proceed to prepare another slide.

Twenty such slides may be prepared in the course of a morning, and when they are finished they should be laid carefully in a cold place, where they will be free from dust. In a week or so the balsam will be quite hard, and then the slide may be completed. Take an old knife, which should be kept for this special purpose; heat the blade in the spirit-lamp, and then run it along the edges of the slide, so as to take off the superfluous balsam which has escaped from beneath the cover. This must be done very quickly, or the balsam inside the cover will be heated by the knife, and the preparation spoiled. When this is done, cut the ornamental paper, as already described, number and label the slide, wash off the ink, and then the preparation is complete. Some objects are very troublesome to prepare, and require to be soaked in turpentine and boiled repeatedly in the balsam before they are completely penetrated with it.

Objects which are put up in Deane’s gelatine are managed after a similar fashion, save that the gelatine is to be heated by placing the bottle in hot water, and that the turpentine is not needed. Vegetable structures show beautifully when thus prepared. To remove the superfluous gelatine use a _wet_ and not a hot knife.

Cells are very difficult to manage, and the novice had better not attempt to make them, but is hereby advised to purchase them ready made. Suppose that the young microscopist has dissected the digestive organs of a bee, and wishes to preserve it in spirit; his best plan will be to use a cell for the purpose. Let him buy a cell of sufficient depth, float the preparation into it, fill it up with spirit, put the cover loosely on, and leave it for a week, occasionally raising the cover and stirring the preparation with a needle, in order to get rid of any air-bubbles that may have been entangled in the tissues.

Then let him wipe the edges of the cell very dry, put on a slight layer of gold-size or asphalte varnish--the former is preferable--fill up the cell a “bumper,” and lay the cover very gently upon it, beginning at one end and gently lowering it. With blotting-paper the liquid that escapes must be removed, the edges dried afresh, a flattened bullet placed on the cover, and with a very small camel’s-hair brush the slightest possible coating of size painted round the edge of the cell. When it has hardened another may be given, and so on, until a thick hard wall of size has been built up round the edges and made the cover completely air-tight.

We presume that the reader does not intend to use his microscope merely as a toy, but that he desires to gain some insight into the works of Nature, and is therefore willing to set to work in a systematic manner.

It is now known that both animal and vegetable structures are built up by means of certain minute particles, technically called CELLS, and that in every part of a plant or of an animal can be recognised the constituents of which it is formed. We will, therefore, begin with the vegetables.

Some of the lowest plants, such as the minute algæ that inhabit the water, afford excellent examples of the simple vegetable cell; but as these plants are not readily procured by a beginner, we will select some familiar object wherein the cells may be found. If any soft and pulpy fruit be taken when it is quite ripe, and submitted to the microscope, the vegetable cell will be seen in a tolerably perfect form. The three rounded objects shown in the accompanying illustration are cells from the strawberry, specimens of which can easily be seen, if a very thin slice be cut with a razor or lancet, the latter being the preferable instrument. Be careful to dip the blade in water before cutting the fruit, and to float the slice from the blade to the glass slide by placing them both under water. Unless this precaution be taken, the section will not be flat, but will be crumpled up, and the cells will not be properly seen.

Within each of these cells may be seen a small rounded object, which is technically called the “nucleus;” and in some cases a smaller nucleus, called the “nucleolus,” may be observed within the nucleus itself. The increase of cells mostly takes place by a process of division. A line passes across the nucleus, which presently separates into two distinct parts, each of which recedes from the other, causing the cell to enlarge and alter its shape. Presently a line is seen across the cell itself, and in due time the cell is also divided into two parts, each having its own nucleus.

In the present instance the cell is totally spherical, because the fruit from which it was taken was soft, and allowed the constituent cells to expand. When, however, the vegetable substance becomes hard, the cells are pressed closely together, and their shapes are very much altered. Sometimes, when the cells are of nearly the same size, and the pressure is equal on every side, the cells form regular twelve-sided figures, called “dodecahedra,” which, when that occurs, show a six-sided outline. A very thin slice of raw potato will show the twelve-sided cells beautifully, and has the further advantage of exhibiting the starch globules with which the cells are filled. Here is a figure of a potato cell, which presents a six-sided outline, just like that of a bee’s waxen dwelling, and which is crowded with the beautiful globules of starch. If the reader likes to make a few dozen balls of clay, and to squeeze them together in a mass, he will find that the central balls will have lost their globular shape, and assumed a more or less regular twelve-sided form, very much like that of the potato cells.

Sometimes the cells run out longitudinally into cylinders, and attain the really enormous length of three inches; sometimes they become flattened, as the skin or epidermis of many plants; and oftentimes they push out their sides into arms or rays, like stars, and form the tissue which is technically called “stellate.” Here is a specimen of stellate tissue taken from the pith of the common rush, wherein the rays are seen to be very regular: generally, however, the rays are extremely irregular, and require some little practice to detect them. Stellate tissue may be seen in the white portion of orange-peel, in the thick fleshy substance of many aquatic plants, in certain leaf-stalks, and in many similar objects.

We will now see how the soft cells which form the pulpy fruit of the strawberry can be changed into the hard timber of the oak or iron-wood tree.

Wherever a cell is destined to form part of a _permanent_ tissue, it is strengthened by receiving certain additions to its walls. These additions are technically known as “secondary deposit,” and are made in various ways. Sometimes they extend in a thin layer over the whole cell-wall, leaving a number of little holes, which are called “pits,” and earning the name of “pitted structures.” Very frequently the secondary deposit is arranged in a series of rings, an example of which is given in the accompanying illustration. This object is taken from the mistletoe. Good examples of the ringed structures may be seen in the anthers of many plants, and in the leaf-stem of the common rhubarb, an example of which is shown in the next illustration. Another very common form of secondary deposit is the spiral, which is generally used where strength and elasticity are united. Two examples of the spiral form are given in the illustration; the first taken from the lily, and the second from the “rhizome,” or subterranean stem of the water-lily.

Another beautiful form of secondary deposit is seen in the fern root. If the root be cut longitudinally, and the dark hard fibre dissolved carefully out with nitric acid, the deposit will seem to have assumed the shape of a winding staircase, and is then called “scalariform,” or ladder-shaped. Similar structures may be found in asparagus.

The reader will see that the hardness of the structure depends entirely on the amount of secondary deposit, and we accordingly find that when the wood is hard and fit to be worked with tools the cells are almost wholly filled with the secondary deposit. In this state they are called “wood-cells.” Examples of these cells may be seen in the accompanying illustration. In the first example, which is taken from the elder-tree, four cells are shown in order to display the manner in which their pointed ends are arranged. (The reader must remember that in all wood-cells the ends are pointed.) In the next example, which is taken from the chrysanthemum, the pitted structure is still retained; but in the last figure, which is drawn from the lime-tree, the entire cell is filled with secondary structure. The reader must understand that we can only give the veriest outline of the subject, and profess to do nothing more than indicate the method of observation, leaving the pupil to work out the details by himself.

Another curious development of the plant-cells is the formation of HAIRS. These objects alone afford an inexhaustible field for the microscopist, and any one who chooses to work out the subject will find himself repaid if he makes a good series of preparations. In their primary forms the hairs are seen merely as little projections on the epidermis, whether of the stem, leaf, or petal, and by degrees assume their varied and beautiful forms. In order to show the singular forms which hairs sometimes assume, an illustration is here given of the hairs of the lavender leaf. This is one of the hairs that give the leaf its silvery gloss. It consists of an upright stem, from the top of which a number of forked branches shoot out horizontally, much like an open umbrella held upright. The object of this remarkable form is, that the delicate vessels in which the perfume is held should escape injury. If the reader will refer to the second figure, which represents a much magnified view of the edge of the leaf, he will see the globular perfume-gland standing under the shelter of the branching hairs.

The following plants afford valuable examples of hair:--Arabis, marvel of Peru, sowthistle, tobacco, southernwood, hollyhock, snapdragon, pansy (in throat of flower), deutzia (under-side of leaf), verbena, alyssum, tradescantia, borage, cowhage, and many others. The beautiful effect produced by the petals of flowers is caused by the imperfect hairs with which their surfaces are studded.

The POLLEN of plants is always worth observing, and some specimens are of remarkably beautiful shapes. Take that of althæa, crocus, cactus, heath, violet, daisy, lily, snowdrop, wallflower, willow-herb (a very beautiful form), hollyhock, periwinkle, primrose, &c. Put some up in Deane’s gelatine, and dry some, besides examining them all when fresh.

The microscopist ought to examine the structures of WOOD by making sections in the directions transverse and longitudinal. A razor will answer very well for the purpose, and the wood should always be soaked inside, and the razor wetted before the section is made. It is often useful to make diagonal sections of several woods, especially those of the pine and juniper. All the forest trees should be examined, and their roots and bark should not be omitted. Cut sections of coconut-shell, vegetable ivory, sugar-cane (a most beautiful object when mounted opaque), bamboo, butcher’s broom, &c.

MOSSES are beautiful objects, and can always be found. Examine particularly the fruit or seed-vessel, and note the structure of its different parts. Put these on a slide, and breathe on them, noting at the same time any change which may take place.

The SPORE CASES of ferns are extremely beautiful, and should be carefully examined. The little brown dots or streaks that are seen on the under surface of the fronds are called “sori,” and contain a large but variable number of the sporanges. These consist of stalked sacs or cases, and differ much in shape, according to the species of fern. If the fern be fresh from which the sorus is taken, the sporanges may be seen writhing and twisting like so many serpents, and sometimes it happens that one of the sporanges bursts, and suddenly covers the field of the microscope with minute black dots. These dots are the spores or seeds of the fern, and when magnified with a very high power, they are seen to be variously shaped. One of the most remarkable spores is that of the equisetum, or mare’s tail of the water. This spore looks like a ball with something coiled round it. As soon as the spore is discharged from its case, four threads are seen to uncoil themselves from around it, and by their elasticity to cause the spore to jump about as if alive. These fibres are technically named elasters, and are prolongations of the outer coat of the spore.

FUNGI of all kinds should be examined. There is never any difficulty in finding fungi, though the autumn is the best time of year for this purpose. “Mould,” as it is popularly called, is a form assumed by many species of fungus, which, though objectionable to the careful housewife, are full of interest to the microscopist. The well-known mushroom and toadstools are the highest of the fungi. The black spots on leaves are fungi, mostly belonging to the genus puccinia, and the best specimens are generally found on the wild rose or bramble. The black “smut” of wheat is another fungus, very pretty under the microscope, but very obnoxious to the farmer; and the “bunt” also belongs to the same vast tribe of plants, four thousand species of which are now known to exist.

The young observer should also look for the beautiful crystals which exist in many vegetable cells. The RAPHIDES, as these crystals are called, are of various forms, mostly shaped like curved needles, but often assuming very pretty and regular outlines. Raphides are plentifully found in the bulb of the onion, in the rhubarb, the lily, the iris, &c. They are best mounted as opaque objects and, if the reader can procure a binocular microscope, he will see the form of the raphides better than with the single-tube instrument.

SEEDS of different plants should be carefully examined, especially those of small dimensions, which often exhibit some wonderful beauties of structure. The winged seed of various plants, such as the thistle, the dandelion, the valerian, and the willow-herb, are extremely interesting objects; while those of the yellow snapdragon, the mullein, the Robin Hood, and the bur-seed, are remarkably beautiful in form, though they have no parachute, as the feathery appendage is called.

Leaving dry land, we will devote a short time to the water. Let the reader take with him the simple collecting apparatus mentioned on page 430, and secure specimens of the water from different ponds, ditches, and streams. For collecting the larger objects a little net, which can be purchased cheap, is of very great use. It is easily made by any tinman, and if the young microscopist knows the use of solder, as all experimental philosophers ought to do, he can put it together in a few minutes. It is formed of a strip of zinc bent into the requisite form, and with a socket, to which a handle can be attached. A piece of coarse muslin, or, rather, fine “net,” is then stretched over the bottom, and the apparatus is complete.

In the water is sure to be found one of the lowest forms of vegetable life--namely, the “confervoid algæ.” Look for these in bright, clear pools, placing the collecting bottle near any greenish film collected around the stems of plants, or spread over the stones on the bed of the pool. If this film be very carefully taken up, it will produce many interesting forms of vegetable life. One of the most remarkable of these vegetables is that which is called “volvox globator,” a figure of which is here given.

This wonderful object is about as large as the head of a very small pin, so that it is visible to the naked eye, and looks like a tiny globule passing through the water. When it is placed under a lens of moderate power, say of an inch focus, it exhibits some very strange peculiarities. It continually revolves, and by its revolution is able to enjoy a moderate degree of locomotion, though without any apparent object. Small dark spots are also seen upon it.

If a half-inch lens be now used, the structure of the volvox begins to be exhibited. The whole surface is covered with a network of very fine fibres, having a spot at the intersection of each mesh. On applying a still higher power, say the four-tenths of an inch, the structure is further elucidated, and the dots on the surface are seen to consist of greenish bodies, each furnished with a pair of delicate fibres, technically named cilia, which are constantly vibrating, and cause the revolution of the general mass. The dark spots are now seen to be the young plants in different stages of progress. From six to ten of these are inclosed within the parent, and when the latter has reached its full age, the membrane bursts asunder, and the little volvoces are liberated.

Another interesting form is the closterium, a genus which is sure to produce several good examples. We may mention that the ponds in Blackheath are very rich in these curious vegetables, and a very considerable series of confervoids may be obtained from them. The closteria are easily recognised by their resemblance to the Australian “boomerang.”

As our space is rapidly waning, we must leave the vegetable, and proceed to the animal kingdom.

As is the case with vegetables, the animal structure is composed of cells, though they cannot be so easily traced as in the examples which we have already noticed. The young observer may readily perceive the animal cell, in its largest and simplest form, by placing a little of the yolk of egg under the microscope. CARTILAGE, or gristle, is easily seen to be composed of cells. The nails of the fingers afford good objects for the microscopist in search of animal cells. If a thin section be placed under the microscope, none but an experienced observer will be able to make out the presence of cells at all; but if the section be soaked in “liquor potassæ,” the cells immediately swell up, and their shape is at once made plain. Take the BONE of a young chicken or rabbit, and make a thin section that embraces both the bone and cartilage, and there will then be a beautiful object for the microscopist, showing how the cartilage is changed by degrees into bone.

Sections of bone should also be made, both transverse and longitudinal.

The BLOOD is another object which must be carefully examined. The “corpuscles” which give the colouring matter to the blood are cells of different size, according to the creature from which they are taken. The dimensions of the animal exercise no apparent influence on the corpuscles, for those of “proteus anguinus,” a little creature not larger than a lamprey, are many times larger than those of the ox. In the accompanying illustration is shown a series of specimens, in order to show the great difference in their shape and size, all being drawn to scale and magnified by the same lens. The circular corpuscles in the left-hand upper corner are those of man; immediately below is a single corpuscle from the pigeon. The great central corpuscle is taken from the proteus; the two in the lower right-hand corner are from the frog, one of these being viewed edgeways; and of the remaining two, that on the left hand belongs to the tortoise, and that on the right to a fish.

The insect tribes are an inexhaustible source of objects for the microscopist, who may find that even a single fly will give him employment for many months. The scales from the butterfly’s wing, the wonderful compound eyes with which insects are gifted, the structure of their feet, and their entire anatomy, are always at the service of any microscopist who really cares for his work. It would, of course, be impossible to give even a list of the interesting portions of the different insects; so one or two examples must suffice us.

Take the ANTENNÆ of the insect tribes, and see how beautifully they are formed, how graceful is the shape, and how elaborate the structure. A low power will be useful for exhibiting their general shape and outline, but it is not until we know how to use the higher powers that the real beauty of these curious organs is seen. In the accompanying illustration is given part of an antenna of the common blue-bottle fly, in order to show the remarkable cavities which exist within the antennæ, and which are thought by some anatomists to be organs of hearing, and by others to be organs of smell.

The WINGS of insects are also most remarkable, and possess many peculiarities of structure which cannot be detected without the aid of a microscope. Take, for example, the wings of any hymenopterous insect, say those of a humble-bee, and see how beautiful is the structure which causes the four wings to be united into two when the insect is about to fly. In the illustration may be seen a pair of these wings, together with the row of hooks which bind them together. A still more magnified representation of the hooks is placed near the wings.

It is now ascertained that the wings of insects are connected with the breathing apparatus, and that the respiration of the insect extends even to the very tips of these singular organs, which are not modifications of existing limbs, as in the birds, but additional structures. The circulation of insects may often be seen by placing a portion of a transparent wing under a moderately high power. We have often seen it in the wing of the great water-beetle. A series of very beautiful preparations may be made in order to show the distinction between the wings of different insects; and as the orders of insects are founded upon their wings, there ought to be at least one example of each order. The proboscis of insects is always worthy of careful examination.

As to the breathing apparatus itself, the best mode of examining it is to open a caterpillar, remove a part of the large breathing tube which runs along each side, and place it under the microscope. It should always be taken so as to include one of the spiracles, or breathing-holes. An example of a breathing-tube, taken from a silk-worm, is given in the illustration.

HAIRS of animals are very curious and interesting objects. They should be mounted in three modes--namely, dry transparent, dry opaque, and in Canada balsam, transparent. Be sure to procure some hair of the bat, the sheep, the mouse, the deer, the mole, and any of the weasel tribe. Many insects have very beautiful hair, but the most lovely hair in the animal kingdom is that which is obtained from the sea-mouse. Fish scales should also be procured, and specimens should be taken from the lateral line.

MOLLUSCS of all kinds afford many beautiful objects, and the observer should be very careful to examine the wonderful tongue-ribbon of the snail, the slug, the periwinkle, the whelk, and other similar molluscs. If meant to be examined by polarized light, the tongue-ribbon should be mounted in Canada balsam.

CRYSTALS should always form part of a collection. Take those of common salt, nitre, sugar, chlorate of potash, salicine, &c.; indeed, anything that will crystallize should be prepared and mounted, as such objects will often be most useful when examining unknown substances.

ZOOPHYTES must of course find a place in the cabinet, and the young microscopist ought to put up a few specimens of the “bird’s-head” processes which are found in the bugularia and other inhabitants of the sea. The pretty noctiluca, to which is mostly owing the phosphorescence of the sea, should be preserved, and the extraordinary appendages to the skin of certain star-fish and sea urchins should be examined. These are called pedicillariæ, and a sketch of them is given in the illustration.

OPTICS AND OPTICAL AMUSEMENTS.

“’Seeing is believing,’ so the sages say,
To prove this false, hear me, my friends, I pray,
And very soon you all will be agreeing,
That nought is so deceptive as our _seeing_.”--MARTIN.

Optics is the science of _light_ and _vision_. Concerning the nature of light, two theories are at present very ably maintained by their respective advocates. One is termed the Newtonian theory, and the other the Huygenean. The Newtonian theory considers light to consist of inconceivably small bodies emanating from the sun, or any other luminous body. The Huygenean conceives it to consist in the undulations of a highly elastic and subtle fluid, propagated round luminous centres in spherical waves, like those arising in a placid lake when a stone is dropped into the water.

LIGHT AS AN EFFECT.

Light follows the same laws as gravity, and its intensity or degree decreases as the square of the distance from the luminous body increases. Thus, at the distance of two yards from a candle we shall have four times less light than we should have, were it only one yard from it, and so on in the same proportion.

REFRACTION.

Bodies which suffer the rays of light to pass through them, such as air, water, or glass, are called refracting media. When rays of light enter these, they do not proceed in straight lines, but are said to be refracted, or bent out of their course, as seen in the drawing. The ray of light proceeding from B through the glass L G is bent from the point C, instead of passing in the direction of the dotted line. But if the ray F C falls perpendicularly on the glass, there is no refraction, and it proceeds in a direct line to K; hence refraction only takes place when rays fall obliquely or aslant on the media.

THE INVISIBLE COIN MADE VISIBLE.

If a coin be placed in a basin, so that on standing at a certain distance it be just hid from the eye of an observer by the rim or edge of the basin, and then water be poured in by a second person, the first keeping his position; as the water rises the coin will become visible, and will appear to have moved from the side to the middle of the basin.

THE MULTIPLYING GLASS.

The multiplying glass is a semicircular piece of glass cut into facets or distinct surfaces; and in looking through it we have an illustration of the laws of refraction, for if a small object, such as a fly, be placed at D, an eye at E will see as many flies as there are surfaces or facets on the glass.

TRANSPARENT BODIES.

Transparent bodies, such as glass, may be made of such form as to cause all the rays which pass through them from any given point to meet in any other given point beyond them, or which will disperse them from the given point. These are called lenses, and have different names according to their form. 1. Is called the plano-convex lens. 2. Plano-concave. 3. Double convex. 4. Double concave. 5. A meniscus, so called from its resembling the crescent moon.

THE PRISM.

The prism is a triangular solid of glass, and by it the young optician may decompose a ray of light into its primitive and supplementary colours, for a ray of light is of a compound nature. By the prism the ray A is divided into its three primitive colours, blue, red, and yellow; and their four supplementary ones, violet, indigo, green, and orange. The best way to perform this experiment is to cut a small slit in a window-shutter, on which the sun shines at some period of the day, and directly opposite the hole place a prism P; a beam of light in passing through it will then be decomposed, and if let fall upon a sheet of white paper, or against a white wall, the seven colours of the rainbow will be observed.

COMPOSITION OF LIGHT.

The beam of light passing through the prism is decomposed, and the spaces occupied by the colours are in the following proportions:--red, 6; orange, 4; yellow, 7; green, 8; blue, 8; indigo, 6; violet, 11. Now, if you paste a sheet of white paper on a circular piece of board about six inches in diameter, and divide it with a pencil into fifty parts, and paint colours in them in the proportions given above, painting them dark in the centre parts, and gradually fainter at the edges, till they blend with the one adjoining. If the board be then fixed to an axle, and made to revolve quickly, the colours will no longer appear separate and distinct, but becoming gradually less visible they will ultimately appear _white_, giving this appearance to the whole surface of the paper.

A NATURAL CAMERA OBSCURA.

The human eye is a camera obscura, for on the back of it on the retina every object in a landscape is beautifully depicted in miniature. This may be proved by the

BULLOCK’S EYE EXPERIMENT.

Procure a fresh bullock’s eye from the butcher, and carefully thin the outer coat of it behind: take care not to cut it, for if this should be done the vitreous humour will escape, and the experiment cannot be performed. Having so prepared the eye, if the pupil of it be directed to any bright objects, they will appear distinctly delineated on the back part precisely as objects appear in the instrument we are about to describe. The effect will be heightened if the eye is viewed in a dark room with a small hole in the shutter, but in every case the appearance will be very striking.

THE CAMERA OBSCURA.

This is a very pleasing and instructive optical apparatus, and may be purchased for four or five shillings. But it may be easily made by the young optician. Procure an oblong box, about two feet long, twelve inches wide, and eight high. In one end of this a tube must be fitted containing a lens, and be made to slide backwards and forwards so as to suit the focus. Within the box should be a plain mirror reclining backwards from the tube at an angle of forty-five degrees. At the top of the box is a square of unpolished glass, upon which from beneath the picture will be thrown, and may be seen by raising the lid A. To use the camera place the tube with the lens on it opposite to the object, and having adjusted the focus, the image will be thrown upon the ground-glass as above stated, where it may be easily copied by a pencil or in colours.

The form of a camera obscura used in a public exhibition is as follows:--D D is a large wooden box stained black in the inside, and capable of containing from one to eight persons. A B is a sliding piece, having a sloping mirror C, and a double convex lens F, which may with the mirror C be slid up or down so as to accommodate the lens to near and distant objects. When the rays proceeding from an object without fall upon the mirror, they are reflected upon the lens F, and brought to fall on the bottom of the box, or upon a table placed horizontally to receive them, which may be seen by the spectator whose eye is at E.

THE CAMERA LUCIDA.

This instrument consists of a glass prism, C, D, D, E, having four sides covered. The sides C, D, being exposed to the object to be delineated, rays pass through the glass and fall on the sloping side D, E; from this they are reflected to the top, and finally pass out of the prism to the eye;[11] now from the direction at which the rays enter the eye, it receives them as if coming from an image at A, B, and if a sheet of paper be placed below the instrument, a perfect delineation of the object may be traced with a pencil. This is a very useful instrument to young draughtsmen.

[11] The eye is to be applied to the little circular hole seen on the
upper surface.

THE MAGIC LANTERN.

This is one of the most pleasing of all optical instruments, and it is used to produce enlarged pictures of objects, which being painted on a glass in various colours are thrown upon a screen or white sheet placed against the wall of a large room. It consists of a sort of tin-box, within which is a lamp, the light of which (strongly reflected by the reflector T,) passes through a great plano-convex lens E fixed in the front. This strongly illuminates the objects which are painted on the slides or slips of glass, and placed before the lens in an inverted position, and the rays passing through them and the lens F, fall on a sheet, or other white surface, placed to receive the image. The glasses on which the figures are drawn are inverted, in order that the images of them may be erect.

PAINTING THE SLIDES.

The slides containing the objects usually shown in a magic lantern, are to be bought at opticians with the lantern, and can be procured cheaper and better in this way than by any attempt at manufacturing them. Should, however, the young optician wish to make a few slides of objects of particular interest to himself, he may proceed as follows:--

Draw first on paper the figures you wish to paint, lay it on the table, and cover it over with a piece of glass of the above shape; now draw the outlines with a fine camel’s hair pencil in black paint mixed with varnish, and when this is dry, fill up the other parts with the proper colours, shading with bistre also mixed with varnish. The transparent colours are alone to be used in this kind of painting.

TO EXHIBIT THE MAGIC LANTERN.

The room for the exhibition ought to be large, and of an oblong shape. At one end of it suspend a large sheet so as to cover the whole of the wall. The company being all seated, darken the room, and placing the lantern with its tube in the direction of the sheet, introduce one of the slides into the slit, taking care to invert the figures; then adjust the focus of the glasses in the tube by drawing it in or out as required, and a perfect representation of the object will appear.

EFFECTS OF THE MAGIC LANTERN.

Most extraordinary effects may be produced by means of the magic lantern; one of the most effective of which is a

TEMPEST AT SEA.

This is effected by having two slides painted, one with the tempest as approaching on one side, and continuing in intensity till it reaches the other. Another slide has ships painted on it, and while the lantern is in use, that containing the ships is dexterously drawn before the other, and represents _ships in the storm_.

The effects of sunrise, moonlight, starlight, &c., may be imitated, also by means of double slides, and figures may be introduced sometimes of _fearful_ proportions.

Heads may be made to nod, faces to laugh; eyes may be made to roll, teeth to gnash; crocodiles may be made to swallow tigers; combats may be represented; but one of the most instructive uses of the slides is to make them illustrative of astronomy, and to show the rotation of the seasons, the cause of eclipses, the mountains in the moon, spots on the sun, and the various motions of the planetary bodies, and their satellites.

THE PHANTASMAGORIA.